OrangerV1, Main, Exploration, bibRecord, 003242

Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids

Identifieur interne : 003242 ( Main/Exploration ); précédent : 003241; suivant : 003243

Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids

Auteurs : R. M. Perez [Espagne] ; O. Mas [Espagne] ; L. Navarro [Espagne] ; N. Duran-Vila [Espagne]

Source :

Plant cell, tissue and organ culture [ 0167-6857 ] ; 1998.

RBID : Pascal:99-0385796

Descripteurs français

Pascal (Inist)
- Production, Cryoconservation, Culture tissu, Végétal, Ovule, Germoplasme, Conservation, Agrume, Cal, Citrus sinensis Citrus reticulata, Citrus deliciosa, Citrus paradisi x Citrus tangerina.
Wicri :
- topic : Production, Agrume.

English descriptors

KwdEn :
- Callus, Citrus fruit, Conservation, Cryopreservation, Germplasm, Ovum, Production, Tissue culture, Vegetals.

Abstract

Assays were performed to obtain embryogenic callus lines from nine mandarin and mandarin hybrid cultivars by in vitro culture of ovules collected from immature fruits six weeks after anthesis. All cultivars produced embryos and loose friable callus. The small proliferations of nucellar callus from cultured ovules were suitable to recover embryogenic callus lines by periodical subculturing to fresh medium. The embryogenic callus cultures were subjected to cryoprotection with 10% (v/v.) (DMSO), freezing by slow cooling, storage in liquid nitrogen and thawing by fast warming. Whole plants from these cryopreserved cultures were recovered through embryogenesis.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000A23
to stream PascalFrancis, to step Curation: 001484
to stream PascalFrancis, to step Checkpoint: 000A50
to stream Main, to step Merge: 003482
to stream Main, to step Curation: 003242

Le document en format XML

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<author><name sortKey="Perez, R M" sort="Perez, R M" uniqKey="Perez R" first="R. M." last="Perez">R. M. Perez</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial</s1>
<s2>46113 Moncada (Valencia)</s2>
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<country>Espagne</country>
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<author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
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<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids</title>
<author><name sortKey="Perez, R M" sort="Perez, R M" uniqKey="Perez R" first="R. M." last="Perez">R. M. Perez</name>
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<author><name sortKey="Mas, O" sort="Mas, O" uniqKey="Mas O" first="O." last="Mas">O. Mas</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial</s1>
<s2>46113 Moncada (Valencia)</s2>
<s3>ESP</s3>
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<s2>46113 Moncada (Valencia)</s2>
<s3>ESP</s3>
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<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
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<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
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<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial</s1>
<s2>46113 Moncada (Valencia)</s2>
<s3>ESP</s3>
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<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
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<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Callus</term>
<term>Citrus fruit</term>
<term>Conservation</term>
<term>Cryopreservation</term>
<term>Germplasm</term>
<term>Ovum</term>
<term>Production</term>
<term>Tissue culture</term>
<term>Vegetals</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Production</term>
<term>Cryoconservation</term>
<term>Culture tissu</term>
<term>Végétal</term>
<term>Ovule</term>
<term>Germoplasme</term>
<term>Conservation</term>
<term>Agrume</term>
<term>Cal</term>
<term>Citrus sinensis  Citrus reticulata</term>
<term>Citrus deliciosa</term>
<term>Citrus paradisi x Citrus tangerina</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Production</term>
<term>Agrume</term>
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<front><div type="abstract" xml:lang="en">Assays were performed to obtain embryogenic callus lines from nine mandarin and mandarin hybrid cultivars by in vitro culture of ovules collected from immature fruits six weeks after anthesis. All cultivars produced embryos and loose friable callus. The small proliferations of nucellar callus from cultured ovules were suitable to recover embryogenic callus lines by periodical subculturing to fresh medium. The embryogenic callus cultures were subjected to cryoprotection with 10% (v/v.) (DMSO), freezing by slow cooling, storage in liquid nitrogen and thawing by fast warming. Whole plants from these cryopreserved cultures were recovered through embryogenesis.</div>
</front>
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<affiliations><list><country><li>Espagne</li>
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<region><li>Communauté valencienne</li>
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<tree><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Perez, R M" sort="Perez, R M" uniqKey="Perez R" first="R. M." last="Perez">R. M. Perez</name>
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<name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
<name sortKey="Mas, O" sort="Mas, O" uniqKey="Mas O" first="O." last="Mas">O. Mas</name>
<name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
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Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids

Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri